Comparison of Ki-67 equivalent antibodies

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Comparison of Ki-67 equivalent antibodies.

AIMS To compare commercially available Ki-67 equivalent antibodies with regard to qualitative and quantitative immunohistochemical staining characteristics. METHODS The following antibodies were used: monoclonal MIB-1 (Immunotech), monoclonal MM1 (Novocastra), polyclonal NCL-Ki-67p (Novocastra), and polyclonal Rah Ki-67 (Dako). All immunostainings were evaluated in squamous epithelium from fo...

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Proliferative activity in human breast cancer: Ki-67 automated evaluation and the influence of different Ki-67 equivalent antibodies

BACKGROUND Ki67 labeling index (Ki67 LI), the percentage Ki67 immunoreactive cells, is a measure of tumor proliferation, with important clinical relevance in breast cancer, and it is extremely important to standardize its evaluation. AIM To test the efficacy of computer assisted image analysis (CAIA) applied to completely digitized slides and to assess its feasibility in routine practice and ...

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Ki - 67 contributes to normal

Department of Molecular, Cell and Cancer Biology 7 University of Massachusetts Medical School, Worcester, MA 01605, USA 8 9 Program in Bioinformatics and Integrative Biology 10 University of Massachusetts Medical School, Worcester, MA 01605, USA. 11 12 Program in Molecular Medicine 13 University of Massachusetts Medical School, Worcester, MA 01605, USA. 14 15 16 Running title: Ki-67 affects S p...

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Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein).

AIMS--To elucidate the fine specificities of the antibodies MIB 1 and MIB 3 and of additional monoclonal antibodies which also recognise the Ki-67 protein (MIB 5, IND.64, JG-67-2a). METHODS--Different parts of the Ki-67 protein cDNA were expressed in Escherichia coli. Bacterial lysates were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and blotted on to nitr...

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Immunoexpression of Ki-67 in pulmonary hypoplasia.

Adequate pulmonary development at birth is the major determinant of postnatal outcome in the perinatal period. Lung hypoplasia is a poorly defined condition. The aim of this study was to investigate expression of Ki-67 in human fetuses with pulmonary hypoplasia compared to fetuses without pulmonary pathology and malformations of other organs used as controls. The analysis comprised 149 formalin...

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 2002

ISSN: 0021-9746

DOI: 10.1136/jcp.55.6.467